A STUDY OF CHEMICAL CARCINOGENESIS .68. EFFECTS OF DIETARY COMPOUNDS ON ALPHA-HYDROXYLATION OF N-NITROSOPYRROLIDINE AND ('-NITROSONORNICOTINE IN RAT TARGET TISSUES

Abstract

Male F344 rats were pretreated with various dietary compounds, and the effects of pretreatment on the in vitro .alpha.-hydroxylation of N-nitrosopyrrolidine or N''-nitrosonornicotine were determined in assays with liver microsomes or cultured esophagus, respectively. Dietary compounds included phenols, cinnamic acids, coumarins, indoles and isothiocyanates. Pretreatments were carried out either by administering the compound by gavage 2 h prior to sacrifice (acute protocol) or by adding the compound to the diet for 2 wk (chronic protocol). Acute pretreatment with benzyl isothiocyanate, allyl isothiocyanate, phenethyl isothiocyanate, phenyl isocyanate and benzyl thiocyanate but not sodium thiocyanate inhibited formation of .alpha.-hydroxylation products of both nitrosamines. When the chronic pretreatment protocol was used, only phenyl isothiocyanate and sodium thiocyanate inhibited formation of .alpha.-hydroxylation products of both nitrosamines. Pretreatments with butylated hydroxyanisole, p-methoxyphenol, or N-acetylcysteine had little, if any, effect on the .alpha.-hydroxylation of N-nitrosopyrrolidine or N''-nitrosonornicotine. Chronic pretreatment with p-hydroxycinnamic acid, 4-hydroxy-3-methoxycinnamic acid, coumarin, umbelliferone, limetine, indole, indole-3-carbinol, indole-3-acetonitrile, and L-tryptophan induced activity for the .alpha.-hydroxylation of N-nitrosopyrrolidine. Isothiocyanates are possible candidates for further study as potential inhibitors of carcinogenesis by N-nitrosopyrrolidine and N''-nitrosonornicotine.