Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes
- 1 September 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 213 (3), 625-633
- https://doi.org/10.1042/bj2130625
Abstract
A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitration and peptide ‘mapping’ experiments demonstrated that transferases D, C and A comprise Yb2Yb2, Yb1Yb2 and Yb1Yb1 subunits.This publication has 24 references indexed in Scilit:
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