Clonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma: Factors influencing growth and Adipogenesis in vitro
- 1 May 1982
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 111 (2), 177-186
- https://doi.org/10.1002/jcp.1041110209
Abstract
We have isolated continuously growing cell lines derived from mouse bone marrow stroma. These cell lines were independently obtained, and though they showed morphologies ranging from the epithelioid to the fibroblastoid patterns, they all differentiated into adipocytes. Subclones obtained from two cell lines had a very high frequency (90‐100%) of differentiation into adipocytes after two or three weeks of arrested growth. Though extensive accumulation of lipid often mechanically impaired mitosis, the cells committed to adipocytes did not suffer an irreversible loss of proliferative capacity. Adipogenesis was obtained in conditions similar to those required for fat cell formation in long‐term bone marrow culture. The cell lines were found to be insensitive to insulin as a signal of adipocyte differentiation. The ultrastructural characteristics of the preadipocytes and fat cells are also similar to those of the fat cells developing in long‐term bone marrow culture. As such, these cell lines should prove useful for analysing cell/cell interactions in haemopoiesis.This publication has 31 references indexed in Scilit:
- Collagen gels as a matrix for haemopoiesisJournal of Cellular Physiology, 1981
- Tunicamycin inhibits the differentiation of ST 13 fibroblasts to adipocytes with suppression of the insulin binding activityBiochemical and Biophysical Research Communications, 1980
- Sensitivity of corticosteroid-dependent insulin-resistant lipogenesis in marrow preadipocytes of obese-diabetic (db/db) miceNature, 1978
- Studies of lipoprotein lipase during the adipose conversion of 3T3 cellsCell, 1978
- Infection of bone marrow cells in vitro with FLV: Effects on stem cell proliferation, differentiation and leukemogenic capacityCell, 1977
- Conditions controlling the proliferation of haemopoietic stem cells in vitroJournal of Cellular Physiology, 1977
- Cellular Interrelationships during in vitro GranulopoiesisDifferentiation, 1976
- An established pre-adipose cell line and its differentiation in cultureCell, 1974
- STROMAL CELLS RESPONSIBLE FOR TRANSFERRING THE MICROENVIRONMENT OF THE HEMOPOIETIC TISSUESTransplantation, 1974
- HEMOPOIETIC COLONY STUDIESThe Journal of Experimental Medicine, 1968