Biosynthesis of C4 acids in Pseudomonas fluorescens KB1

Abstract
Evidence is presented that the biosynthesis of aspartic acid in glucose-grown Pseudomonas fluorescens KB 1 cells relies heavily, if not exclusively, on the operation of fixation of carbon dioxide of the C1 + C3 type. The C3 unit, presumably of the nature of pyruvate, is believed to be derived from glucose via the Entner-Doudoroff pathway, which accounts for the catabolism of nine-tenths of the substrate glucose in this organism.