1,25-DIHYDROXY-VITAMIN-D3 - INVIVO AND INVITRO EFFECTS ON HUMAN PRELEUKEMIC AND LEUKEMIC-CELLS
- 1 December 1985
- journal article
- research article
- Vol. 69 (12), 1399-1407
Abstract
The active metabolite of vitamin D known as 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] is a major physiologic regular of mineral metabolism in man. The compound is also a potent inducer of differentiation of a human promyelocytic leukemia cell line known as HL-60. The induction of differentiation of myeloid leukemia cells to functional end cells offers an appealing therapeutic prospect. We investigated the ability of 1,25(OH)2D3 both to induce in vitro the differentiation of blast cells taken from patients with acute myelogenous leukemia and to improve hematopoiesis in vivo in patients with the myelodysplastic syndromes (preleukemia). We found that high concentrations (10-6 M) of 1,25(OH)2D3 significantly induced the in vitro differentiation of blast cells as measured by morphology, phagocytosis, and superoxide production. A concentration of 10-9 M 1,25(OH)2D3 had no effect on blast cell differentiation. We gave 2 .mu.g/day of 1,25(OH)2D3 to 18 patients with meylodysplastic syndrome (preleukemia) in an attempt to improve their hematopoiesis. During therapy, their peak peripheral blood granulocyte, platelet, and macrophage concentrations were slightly elevated as compared to their baseline, starting levels. Eight patients had a partial or minor peripheral blood resposne to the compound during the administration of 1,25(OH)2D3. However, no patient showed significant improvement of peripheral blood cell or marrow blast cell counts by the end of the study (.gtoreq. 12 weeks) as compared to their starting levels. Seven of the patients developed leukemia before or by 12 weeks of tratment. Nine of the 18 patients developed hypercalcemia. Taken together, the study shows that high concentrations (10-6 M) of 1,25(OH)2D3 can induce differentiation of leukemic blast cells in vitro, but the administration of 1,25(OH)2D3 to patients with the myelodysplastic syndromes (preleukemia) does not have an enduring therapeutic effect. Hypercalcemia prevented administering greater amounts of 1,25(OH)2D3. In the future, the use of new vitamin D analogs that induce hematopoietic cell differentiation without inducing hypercalcemia might allow the achievement of higher blood levels of the inducing compound and might be medically useful for selected preleukemic and leukemic patients.This publication has 14 references indexed in Scilit:
- VARYING INVOLVEMENT OF PERIPHERAL GRANULOCYTES IN THE CLONAL ABNORMALITY - 7 IN BONE-MARROW CELLS IN PRELEUKEMIA SECONDARY TO TREATMENT OF OTHER MALIGNANT-TUMORS - CYTOGENETIC RESULTS COMPARED WITH RESULTS OF FLOW CYTOMETRIC DNA ANALYSIS AND NEUTROPHIL CHEMOTAXISCE1982
- Effects of calcitriol administration on calcium metabolism in healthy menKidney International, 1982
- 1α,25-Dihydroxyvitamin D3 induces differentiation of human myeloid leukemia cellsBiochemical and Biophysical Research Communications, 1981
- Differentiation of mouse myeloid leukemia cells induced by 1 alpha,25-dihydroxyvitamin D3.Proceedings of the National Academy of Sciences, 1981
- Human PreleukemiaAnnals of Internal Medicine, 1980
- Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid.Proceedings of the National Academy of Sciences, 1980
- Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemiaNature, 1978
- Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds.Proceedings of the National Academy of Sciences, 1978
- CELLULAR MATURATION IN HUMAN PRELEUKEMIA1978
- Proposals for the Classification of the Acute Leukaemias French‐American‐British (FAB) Co‐operative GroupBritish Journal of Haematology, 1976