A Computer Program for Analysis of Clotting Factor Assays and Other Parallel-line Bioassays

Abstract

A computer program for the analysis of coagulation factor assays is presented. It can be run on small computers utilizing FORTRAN. It allows considerable flexibility in assay design, accommodates any sample dilution scheme, different numbers of standard and test doses, and missing data points. The report lists the input data for each assay, the analysis of variance for the regression lines, and the potency ratio, as well as statistics that allow estimates of the precision of the potency ratio. It is suggested that interpretation of clotting factor assay results would be facilitated if reports regularly included such information about the reliability of the potency ratio measurements.