Ethylene production from α-oxo-γ-methylthiobutyric acid is a sensitive measure of ligninolytic activity by Phanerochaete chrysosporium
- 15 August 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 206 (2), 423-425
- https://doi.org/10.1042/bj2060423
Abstract
Production of hydroxyl radical in ligninolytic cultures was determined by measuring the .alpha.-oxo-.gamma.-methylthiobutyric acid-dependent production of ethylene gas. The pattern of ethylene production was very similar to that of ligninolytic activity ([14C]lignin .fwdarw. 14CO2). Nutritional parameters, which affect ligninolytic activity, affected OH.-radical-dependent ethylene production in a similar fashion. Apparently, assay for ethylene production from .alpha.-oxo-.gamma.-methylthiobutyric acid is a simple and sensitive measure of ligninolytic activity by P. chrysosporium.This publication has 7 references indexed in Scilit:
- Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporiumApplied and Environmental Microbiology, 1981
- Keratoglobus and complicated microphthalmos.1978
- THE GENERATION OF HYDROXYL RADICALS IN BIOLOGIC SYSTEMS: TOXICOLOGICAL ASPECTSPhotochemistry and Photobiology, 1978
- Ligninolytic enzyme system of Phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvationJournal of Bacteriology, 1978
- Preparation and microbial decomposition of synthetic [14C]ligins.Proceedings of the National Academy of Sciences, 1975