A MODIFIED TURBIDIMETRIC METHOD FOR THE ASSAY OF HYALURONIDASE

Abstract

With the possible exception of the turbidimetric assay, no reliable or practical method exists for the accurate determination of the potency of hyaluronidase preparations. Although the turbidimetric method is by no means ideal, it does lend itself to the performance of a large series of tests and it is accurate to within 10 per cent (Meyer, 1947). A modification of the turbidimetric hyaluronidase assay is being used in this laboratory with good results. It is based on the turbidimetric method las developed by Seastone (1939, 1943), Kass and Seastone (1944), and Leonard, Perlman and Kurzrok (1946). The modification in assay procedure is the result of the daily handling for approximately eleven months of 10 to 20 assays under practical working conditions. With careful attention to details, the method is accurate to within ±5 per cent. materials and methods Standard hyaluronidase preparations. In order to minimize day-to-day deviation of assay values, a dry hyaluronidase preparation (bull testis) is set aside as the standard enzyme.