Prevention of SV40 Virus Tumorigenesis by Irradiated, Disrupted and Iododeoxyuridine Treated Tumor Cell Antigens.

Abstract

Hamster SV40 [Simian virus 40] tumor cells rendered nonproliferative by exposure to gamma rays or by propagation in the presence of iododeoxyuridine were highly effective when used as immunizing antigens for preventing the appearance of tumors in hamsters which had received SV40 virus when newborn. Only a single infection of immunizing antigen was employed. Disruption of tumor cell prepara-tions by freeze-thaw or by treatment in a French pressure cell for purpose of fractionation resulted in total or near total loss of immunizing capability, even when incorporated into alum adjuvant. Possible mechanisms for loss of potency are discussed.