METABOLISM OF 2-METHYLNAPHTHALENE BY RAT AND RAINBOW-TROUT HEPATIC MICROSOMES AND PURIFIED CYTOCHROMES-P-450
- 1 January 1985
- journal article
- research article
- Vol. 13 (5), 542-547
Abstract
The metabolism of 14C-2-methylnaphthalene by hepatic microsomes and purified cytochromes P-450 from rat and rainbow trout was studied using HPLC and radioactivity counting. The effects of pretreatment with .beta.-naphthoflavone and phenobarbital on the formation of three dihydrodiol metabolites and 2-hydroxymethylnaphthalene by microsomes and purified cytochromes P-450 were compared. The metabolism observed with microsomes could not adequately be accounted for by the combined metabolism observed with the purified cytochromes P-450. Differences in the microsome suspension buffers used when prepared microsomes for cytochrome purification as compared to preparing microsomes for direct use appeared to be a significant factor in these differences.This publication has 13 references indexed in Scilit:
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