Lack of Association of Serologically Detectable Human Melanoma-Associated Antigens With Beta2 Microglobulin: Serologic and Immunochemical Evidence23
- 1 October 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 65 (4), 703-707
- https://doi.org/10.1093/jnci/65.4.703
Abstract
Serologic and immunochemical assays showed that human melanoma-associated antigens (MAA) identified with operationally specific xenoantisera were neither spatially nor structurally associated with β2-microglobulin (β2-μ), the light chain of the HLA-A,B antigen molecular complex; i.e., cultured melanoma cells coated with a specific anti-β2-μ xenoantiserum maintained their reactivity with anti-MAA xenoantisera. Furthermore, soluble MAA were not bound by a β2-μ immunoadsorbent. Finally, MAA were shed into the culture medium of melanoma cells and then were immunoprecipitated with specific anti-MAA xenoantisera; analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, they appeared as two distinct structures with molecular weights of 240,000 and 94,000 but comprised no structure with the characteristic 12,000 molecular weight of β2-μ. Conversely, immunoprecipitates obtained by the reaction of spent culture medium of [3H]valine-labeled melanoma cells with anti-β2-μ xenoantiserum had the 12,000-molecular-weight component but no structures with the molecular weights established for MAA. Thus the data refute the contention that serologically detectable MAA have a molecular structure similar to that of HLA antigens.Keywords
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