Molecular analysis of the rfb gene cluster of Salmonella serovar muenchen (strain M67): the genetic basis of the polymorphism between groups C2 and B
- 1 May 1992
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (10), 1385-1394
- https://doi.org/10.1111/j.1365-2958.1992.tb00859.x
Abstract
The rfb (O antigen) gene cluster of group C2 Salmonella differs from that of group B in a central region of 12.4kb: we report the sequencing of this region of strain M67 (group C2) and a subsequent comparison with the central region of strain LT2 (group B). We find a block of seven open reading frames unique to group C2 which encode the O antigen polymerase (rfc) and the transferases responsible for assembly of the group C2 O antigen. The remaining rfb genes are common to strains M67 and LT2, but rfbJ (GDP‐abequose synthase) and rfbM and rfbK (GDP‐mannose synthesis), which are immediately adjacent to the central region, are highly divergent. All these genes have a low G+C content and appear to have been recent additions to Salmonella enterica. We discuss the evolutionary significance of the arrangement and divergence of the genes in the polymorphism of the rfb cluster.Keywords
This publication has 35 references indexed in Scilit:
- Cloning of the rfb gene cluster of a group C2 Salmonella strain: comparison with the rfb regions of groups B and DMolecular Microbiology, 1991
- Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)Molecular Microbiology, 1991
- The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutantsNature, 1989
- The detection and classification of membrane-spanning proteinsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Identification of common molecular subsequencesJournal of Molecular Biology, 1981
- Nucleotide sequences of the trpG regions of Escherichia coli, Shigella dysenteriae, Salmonella typhimurium and Serratia marcescensJournal of Molecular Biology, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970