Further Characterization and Evidence for a Precursor in the Formation of Plasma Antinatriferic Factor

Abstract

Antinatriferic factor was isolated from VE [extracellular fluid volume expanded] dog plasma on high pressure liquid chromatography. The use of an enzyme inhibitor while collecting plasma reduced the presence of this factor. A reverse-phase chromatography peptide map revealed 2 peptides whose presence was directly correlated with antinatriferic activity. Antinatriferic factor is apparently a small acidic peptide, formed from a precursor molecule. Reverse-phase chromatography may prove to be a chemical assay for antinatriferic factor.