Studies on Polyamines. I. A New Fluorometric Determination of Spermine and Spermidine.

Abstract

A new fluorometric method for the estimation of spermine and spermidine was devised on the basis of the findings that one of the oxidation products of spermine and spermidine by beef plasma amine oxidase reacts with resorcinol to produce a fluorescent compound. It was also attempted to estimate spermidine in biological materials by the combination of a simple separation procedure with the specific assay method presented in this investigation.