A wheat histone H3 promoter confers cell division‐dependent and ‐independent expression of the gus A gene in transgenic rice plants
- 1 February 1993
- journal article
- Published by Wiley in The Plant Journal
- Vol. 3 (2), 241-252
- https://doi.org/10.1046/j.1365-313x.1993.t01-16-00999.x
Abstract
To investigate developmental regulation of wheat histone H3 gene expression, the H3 promoter, which has its upstream sequence to -1711 (relative to the cap site as +1), was fused to the coding region of the gus A gene (-1711H3/GUS) and introduced into a monocot plant, rice. Detailed histochemical analysis revealed two distinct types of GUS expression in transgenic rice plants; one is cell division-dependent found in the apical meristem of shoots and roots and in young leaves, and another is cell division-independent detected in flower tissues including the anther wall and the pistil. In this study, replication-dependent expression occurring in non-dividing cells which undergo endoreduplication could not be discriminated from strict replication-independent expression. The observed expression pattern in different parts of roots suggested that the level of the H3/GUS gene expression is well correlated with activity of cell division in roots. To identify 5' sequences of the H3 promoter necessary for an accurate regulation of the GUS expression, two constructs containing truncated promoters, -908H3/GUS and -185H3/GUS, were analyzed in transiently expressed protoplasts, stably transformed calli and transgenic plants. The results indicated that the region from -909 to -1711 contains the positive cis-acting element(s) and that the proximal promoter region (up to -185) containing the conserved hexamer, octamer and nonamer motifs is sufficient to direct both cell division-dependent and -independent expression. The use of the meristem of roots regenerated from transformed calli for the analysis of cell division-dependent expression of plant genes is discussed.Keywords
This publication has 49 references indexed in Scilit:
- Highly conserved hexamer, octamer and nonamer motifs are positive cis‐regulatory elements of the wheat histone H3 geneFEBS Letters, 1992
- Sequence-specific single-strand DNA-binding proteins that interact with the regulatory regions of wheat histone H3 and H4 genesBiochemical and Biophysical Research Communications, 1991
- Multiplicity of the DNA‐binding protein HBP‐1 specific to the conserved hexameric sequence ACGTCA in various plant gene promotersFEBS Letters, 1989
- Function of the hexameric sequence in the cauliflower mosaic virus 35S RNA promoter regionBiochemical and Biophysical Research Communications, 1989
- A Protein That Binds to a cis-acting Element of Wheat Histone Genes Has a Leucine Zipper MotifScience, 1989
- Spatial patterns of histone mRNA expression during grain development and germination in riceCell Differentiation and Development, 1989
- DNA‐binding protein(s) interacts with a conserved nonameric sequence in the upstream regions of wheat histone genesFEBS Letters, 1988
- Nuclear protein(s) binding to the conserved DNA hexameric sequence postulated to regulate transcription of wheat histone genesFEBS Letters, 1987
- Genomic organization and nucleotide sequences of two corn histone H4 genesGene, 1986
- An Analysis of Cell Morphology and the Periodicity of Division in the Root Tip of Allium CepaAmerican Journal of Botany, 1958