Characterisation of γ‐glutamylcysteine synthethase‐heavy subunit promoter: a critical role for AP‐1
- 1 May 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 427 (1), 129-133
- https://doi.org/10.1016/s0014-5793(98)00410-4
Abstract
The 5′-flanking region of human γ-glutamylcysteine synthetase-heavy subunit (γ-GCS-HS) was characterised by creating a series of chloramphenicol acetyl transferase (CAT) reporter deletion constructs. Analysis of various deleted CAT constructs revealed that a putative AP-1 consensus sequence is required to direct the constitutive and oxidant-mediated promoter activity. Gel mobility shift and mutation analysis of the sequence (−269 to −263 bp), showed binding of AP-1 is involved in the oxidant-mediated regulation of γ-GCS-HS promoter activity.Keywords
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