A New Monoclonal Antibody Recognizing an Antigen of Human Lymphocytes Similar or Identical to Tac Antigen
- 1 September 1984
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 28 (9), 1041-1055
- https://doi.org/10.1111/j.1348-0421.1984.tb00760.x
Abstract
A new mouse monoclonal antibody (HEI, IgG1 type) that reacts with a cell surface glycoprotein of human lymphocytes was isolated. Membrane immunofluorescence assay showed that HIEI, like the anti-Tac monoclonal antibody, reacted preferentially with activated normal human T-cells and adult T-cell leukemia virus (ATLV)-carrying human T- and B-cell lines. However, an interesting difference between HIEI and anti-Tac antibody was that HIEI did not react with ATLV-transformed simian cell lines or those cultured with interleukin-2 (IL-2), whereas the anti-Tac antibody did. The immunoprecipitation assay showed that both HIEI and anti-Tac antibody precipitated a glycoprotein with a MW of 60,000 daltons (gp60) from activated normal T-cells and ATLV-positive T- and B-cells and also gp53 from MT-2 and MT-2-related T-cell lines transformed with ATLV in vitro by the MT-2 cocultivation method. HIEI inhibited the IL-2-dependent proliferation of normal T-cells, but its inhibitory effect was much weaker than that of the anti-Tac antibody. The anti-Tac antibody interfered with the binding of HIEI to target cells, but HIEI did not block binding of the anti-Tac antibody to the cells. These observations indicate that HIEI antibody recognizes a new antigenic determinant of the human Tac antigen.This publication has 45 references indexed in Scilit:
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