Über die proteolytischen Enzyme der Milz. (10. Mitteilung zur Spezifität tierischer Proteasen.)

Abstract

S. G. Hedin had shown that the spleen contains 3 proteolytic enzymes[long dash]2 proteases with optimum reactions at ph 4.0 and 8.0 respectively and a peptidase of the erepsin type. By treating one fraction of a glycerin extract of the fresh organs with an electronegative adsorbing substance (diatomaceous earth or kaolin) the protease with 2 peaks, acid and alkaline, is obtained. Treatment of the glycerin extract with basic alumina removes the erepsin component. With the erepsin removed the akalaline reaction of the protease disappears, while the acid remains. It is concluded that there are only 2 proteolytic enzymes in the spleen, a protease with optimum reaction at pH 4.0, specific for the hydrolysis of proteins, and, in an overwhelming quantity, a peptid-splitting enzyme with an optimum reaction at pH 8.0.