Expression in animal cells of the 5‐HT1A receptor by a vaccinia virus vector system
- 27 April 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 301 (3), 303-306
- https://doi.org/10.1016/0014-5793(92)80262-f
Abstract
The co-infection or infection—transfection variants of the T7 RNA polymerase/vaccinia vector system were used to express 5-HT1ARs in COS-7, BSC-40 and GH3 cells, with co-infection giving ca. 3-fold higher level than infection—transfection. Binding affinities were similar to those of the endogenous 5-HT1AR, with highest affinities for 5-HT and 8-OH-DPAT. Functional properties were demonstrated by assays of agonist-stimulated GTPase activity and its inhibition by pertussin toxin. Immunoblot assays showed expression of the unglycosylated and glycosylated receptor protein in the membrane and, surprisingly, in the cytosolic fractions.Keywords
This publication has 15 references indexed in Scilit:
- Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.Proceedings of the National Academy of Sciences, 1991
- Vaccinia Virus: A Tool for Research and Vaccine DevelopmentScience, 1991
- 5-HT Receptors: Subtypes and Second MessengersJournal of Receptor Research, 1991
- Ionic Channels and Their Regulation by G Protein SubunitsAnnual Review of Physiology, 1990
- Functional expression of Shaker K+ channels in a baculovirus-infected insect cell lineNeuron, 1990
- Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.Proceedings of the National Academy of Sciences, 1989
- The genomic clone G-21 which resembles a β-adrenergic receptor sequence encodes the 5-HT1A receptorNature, 1988
- An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteinsNature, 1987
- Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.Molecular and Cellular Biology, 1987
- Catecholamine-stimulated GTPase activity in turkey erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Enzymology, 1976