The effect of gamma irradiation on injectable human amnion collagen
- 1 August 1989
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 23 (8), 833-844
- https://doi.org/10.1002/jbm.820230803
Abstract
The effect of gamma irradiation on the physicochemical properties of injectable human amninon collagen was investigated. Pepsin‐extracted human amnion collagen was purified, reconstituted, and irradiated with varying doses of gamma irradiation (0.25 Mrads to 2.5 Mrads). Gamma irradiation had a significant impact on the physical characteristics of the collagen. The neutral solubility of collagen in PBS at 45°C was decreased from 100% for the nonirradiated control sample to 16% for the 2.5 Mrads irradiated sample. SDS polyacrylamide gel electrophoresis also demonstrated the dose‐dependent effect of gamma irradiation on collagen crosslinks. Electron microscopic observation revealed that even at low irradiation dose (0.25 Mrads), collagen fibril diameter increased. The average diameter was 50 nm for nonirradiated control fibrils, while 4.4% percent of the irradiated collagen fibrils had a diameter greater than 100 nm. Irradiated collagen showed little evidence of damage. Well‐preserved cross‐strations were found in collagen fibrils at all doses of irradiation. Native amnion collagen irradiated with gamma rays demonstrated a slight increase in resistance to collagenase degradation compared with nonirradiated native collagen samples. Increased resistance to collagenase did not correlate with increasing irradiation dose. After 30 min of incubation at 37°C, both irradiated and non‐irradiated collagen was completely digested by collagenase. However, gamma‐irradiated collagen did become more sensitive to hydrolysis by trypsin. The higher the irradiation doses used, the greater sensitivity to trypsin was observed. At 0.25 Mrads irrdiation only a slight increase was found. No marked differences in amino acid composition were noted among the high dose irradiated, low dose irradiated and control amnion collagen.This publication has 13 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- The preparation and physicochemical characterization of an injectable form of reconstituted, glutaraldehyde cross‐linked, bovine corium collagenJournal of Biomedical Materials Research, 1986
- Electron microscopy of the collagen fibrilPublished by Springer Nature ,1984
- A simple method to determine nanogram levels of 4-hydroxyproline in biological tissuesAnalytical Biochemistry, 1981
- CollagenPublished by Springer Nature ,1981
- The estimation of two collagens from human dermis by interrupted gel electrophoresisBiochemical and Biophysical Research Communications, 1976
- Collagen Polymorphism: Characterization of Molecules with the Chain Composition [α1(III)] 3 in Human TissuesScience, 1974
- Analysis of bacteriophage T7 early RNAs and proteins on slab gelsJournal of Molecular Biology, 1973
- Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole RangeScience, 1972
- Effects of Low-dosage Gamma-radiation on Soluble CollagenNature, 1964