POLYACRYLAMID-GEL-ELECTROPHORESIS OF HEMOPHILUS PROTEINS

Abstract

After phenol-acetic-acid extraction the following Haemophilus strains were subjected to polyacrylamide-gel-electrophoresis in presence of 8 M urea: strains of the serovar A of H. paragallinarum-0083, 1516, 1598, 2213, 1645, 1646, Lorehren, 2671, 1385, 758, 17756; strains of serovar B of H. paragallinarum-0222, 2600, 733, 2028, 1596, 2026, 1676, 245, the S and R-form of 2403 and the strains 782 and 1655, which were not serotyped; strains of H. paravium 1762, 62 (Serovar 1), 2654, 2659 (Serovar 2), 780 (Serovar 3), 94 (Serovar 4) and 1254, 0002, 0003, which were not serotyped. H. parainfluenzae (NCTC 4101) and H. parasuis were examined in the same way. The Coomassie Blue-stained protein patterns show that each of the strains tested developed its characteristic protein pattern, with exception of the S- and R-form of the strain 2403, which developed identical pattern. Interrelations between electrophoretic pattern and biological properties such as biochemical activities or pathogenicity could not be proved. The procedure described seems to be suitable for strain- or clone-identification on the subspecies level. The electrophoresis apparatus was less expensive than corresponding available equipments and was usable for the polyacrylamide-gel-electrophoresis.