Development of membrane interactions between brain endothelial cells and astrocytes in vitro
- 1 January 1988
- journal article
- research article
- Published by Wiley in International Journal of Developmental Neuroscience
- Vol. 6 (1), 25-37
- https://doi.org/10.1016/0736-5748(88)90026-3
Abstract
To ascertain whether there is a mutual influence on the structure of their cell membranes, brain endothelial cells and their closest neighbor, astrocytes, were grown alone or together in vitro and freeze-fractured. When cultured separately, the brain endothelial cells had a low frequency of short, fragmented tight junctions. Many gap junctions, which are absent from mature brain capillaries in vivo, intercalated among the tight junctional strands, or were separate from them. The separately cultured astrocytes had low concentrations of randomly distributed assemblies (1–30/μm2) in their membranes. When the two cell types were co-cultured, the endothelial tight junctions were greatly enhanced in frequency, length, width and complexity, and the gap junctional area enclosed by the tight junctional strands were markedly reduced. Thus, the in vitro endothelial junctional complex resembled their in vivo counterpart, the tight junctions of brain capillaries, when co-cultured with astrocytes. Reciprocally, brain endothelial cells induced the astrocytic membrane assemblies to increase in concentrations by 5̃ fold, and sometimes to form aggregates with very high concentrations (400/μm2) which approached the concentration of the perivascular astrocytic membranes in vivo. Substituting astrocytes with fibroblasts or smooth muscle cells in co-cultures did not enhance the tight junctions in the brain endothelium. On the other hand, substituting brain endothelium with endothelium from pulmonary artery or aorta in cocultures did not increase the concentration or induce aggregation of the assemblies in the astrocytes. Thus, the two close neighbors in vivo, brain endothelium and astrocytes, interact specifically in vitro to induce development of membrane specializations which resemble those at the site of the blood-brain barrier.Keywords
This publication has 26 references indexed in Scilit:
- Brain microvessel endothelial cells in tissue culture: A model for study of blood‐brain barrier permeabilityAnnals of Neurology, 1983
- Structural integrity of hepatocyte tight junctions.The Journal of cell biology, 1983
- Particle assemblies in astrocytic plasma membranes are rearranged by various agentsin vitro and cold injuryin vivoJournal of Neurocytology, 1982
- Rapid Massive Assembly of Tight Junction StrandsScience, 1981
- Developing nervous tissue induces formation of blood-brain barrier characteristics in invading endothelial cells: A study using quail-chick transplantation chimerasDevelopmental Biology, 1981
- Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.The Journal of cell biology, 1980
- Junction formation in trypsinized cells of human adenocarcinoma cell lineExperimental Cell Research, 1978
- Freeze‐fracture appearance of the capillary endothelium in the cerebral cortex of mouse brainJournal of Anatomy, 1974
- ARRAYS OF PARTICLES IN FREEZE-FRACTURED ASTROCYTIC MEMBRANESThe Journal of cell biology, 1974
- Pronase Effect on Pancreatic Beta Cell Secretion and MorphologyScience, 1973