The Production of a State of Active Immunity to Isologous and Autologous Serum Proteins

Abstract

Acute hypogammaglobulinemla and hypoalbuminemia of varying duration and severity were produced in dogs by inducing an active state of immunity against isologous [gamma]-globulin or autologous albumin. This was accomplished by coupling isologous dog [gamma]-globulin or autologous dog albumin to a highly antigenic protein (human [gamma]-globulin) by means of a bisdlazobenzidine linkage, and reintroducing these complexes into dogs. The presence of specific antibody to dog [gamma]-globulin and albumin was demonstrated in a number of the hypogammaglobu-linemic and hypoalbuminemic animals, thereby indicating that these experimental results had an immunologic basis.