Signalling through the leukotriene B4 receptor involves both αi and α16, but not αq or α11 G-protein subunits
- 1 October 1998
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 335 (1), 15-18
- https://doi.org/10.1042/bj3350015
Abstract
COS-7 cells transfected with the leukotriene (LT) B4 receptor (BLTR) cDNA were unable to produce LTB4-induced inositol phosphates (IPs) in spite of the presence of endogenous Gαi, Gαq and Gα11 proteins. Co-transfection of BLTR with Gα16, however, resulted in high levels of IP production, which were 17-, 10- and 6-fold higher than with co-transfected Gα11, Gαq and Gα14, respectively. Co-transfection of BLTR with phospholipase C (PLC) β2, on the other hand, resulted in efficient IP production and co-transfection of BLTR with both Gα16 and PLCβ2 resulted in a greater than additive response.Keywords
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