β‐oxidation of polyunsaturated fatty acids in peroxisomes Subcellular distribution of Δ3,Δ2‐enoyl‐CoA isomerase activity in rat liver
- 11 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 215 (2), 228-232
- https://doi.org/10.1016/0014-5793(87)80151-5
Abstract
The metabolism of the double bonds at the Δ3 position in fatty acids was studied in rat liver. Infusion of δ3-trans-dodecenoic acid into isolated perfused liver and subecllular fractionation studies showed the presence of both peroxisomal and mitochondrial Δ3,Δ2-enoyl-CoA isomerase activity (EC 5.3.3.8). These findings together with the previous demonstration of peroxisomal 2,4-dienoyl-CoA reductase (EC 1.3.1.34) [(1981) J. Biol. Chem. 256, 8259–8262] and D-3-OH-acyl-CoA epimerase (EC 5.1.2.3) [(1985) FEBS Lett. 185, 129–134] activities show that peroxisomes possess all the auxiliary enzymes required for the β-oxidation of unsaturated fatty acids.Keywords
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