Increased sensitivity for Coomassie staining of sodium dodecyl sulfate‐polyacrylamide gels using PhastSystem Development Unit
- 1 January 1988
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 9 (1), 60-61
- https://doi.org/10.1002/elps.1150090112
Abstract
Staining of proteins in PhastGel gradient media with Coomassie Blue R 350 was considerably improved using a lower concentration of methanol (10% v/v) and 2% ammonium sulfate in the staining solution and 10% acetic acid for destaining. The detection limit in sodium dodecyl sulfate-polyacrylamide gels was lowered by a factor of 10 to about 2 ng per protein band. The Coomassie staining method was adapted to the newly developed silver staining procedure so that both can be used in parallel in PhastSystem.This publication has 4 references indexed in Scilit:
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- Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver stainingElectrophoresis, 1985
- Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysisElectrophoresis, 1985
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971