Purification of the cytosol oestradiol-receptor complex from foetal guinea-pig uterus using electrofocusing on polyacrylamide plates
- 1 February 1978
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 34 (2), 268-269
- https://doi.org/10.1007/bf01944721
Abstract
The3H-oestradiol receptor complex obtained from the cytosol fraction of foetal guinea-pig uterus was purified by the following steps: column chromatography in Sephadex G-15 and Ultrogel, and by electrofocusing on polyacrylamide plates. In the final step a concentration of 15–17% of the foetal uterine oestradiol receptor protein was obtained. The isoelectric point (pl) of this receptor was determined to be 6.1–6.2.Keywords
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