Cyclic Nucleotide-Dependent Protein Kinase Modulators in Thyroid Tissue*

Abstract

A heat-stable protein which inhibits cAMP dependent protein kinase was prepared from the bovine thyroid 100,000 × g supernatant. This protein inhibited the cAMPdependent protein kinase both from bovine thyroid cytosol and bovine thyroid plasma membranes. The inhibitory effect was noncompetitive with histone as substrate of the cytosolic enzyme. The stimulatory modulator for cGMP-dependent protein kinase was separated from the 100,000 × g supernatant by Sephadex G-100 gel filtration. The stimulatory modulator had no stimulating effect on cAMP-dependent phosphorylation, and the inhibitory modulator of cAMP-dependent enzyme had no effect on cGMP-requiring phosphorylations. The inhibitory modulator may regulate cAMP-dependent protein kinase activity in cytosol and plasma membrane, and the stimulatory modulator for cGMP-dependent protein kinase may have a role in thyroid function independent of the cAMP-requiring system. (Endocrinology 105: 204, 1979)