Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation
Open Access
- 18 March 2012
- journal article
- research article
- Published by Hindawi Limited in Journal of Allergy
- Vol. 2012, 1-13
- https://doi.org/10.1155/2012/819176
Abstract
Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis.Keywords
Funding Information
- Hospital Research Foundation (519206)
This publication has 35 references indexed in Scilit:
- Detection of immunological biomarkers correlated with asthma control and quality of life measurements in sera from chronic asthmatic patientsAnnals of Allergy, Asthma & Immunology, 2011
- Modelling asthma in macaques: longitudinal changes in cellular and molecular markersEuropean Respiratory Journal, 2010
- Inflammasome and IL-1β-Mediated DisordersCurrent Allergy and Asthma Reports, 2010
- NLRP3 inflammasome activation: the convergence of multiple signalling pathways on ROS production?Nature Reviews Immunology, 2010
- ATP-dependent activation of an inflammasome in primary gingival epithelial cells infected byPorphyromonas gingivalisCellular Microbiology, 2010
- Monocyte Derived Microvesicles Deliver a Cell Death Message via Encapsulated Caspase-1PLOS ONE, 2009
- Mechanisms of interleukin-1β releaseImmunobiology, 2009
- Serum interleukin-1β as a marker for differentiation of asthma and chronic obstructive pulmonary diseaseBiomarkers, 2008
- Orchestration of pathogen recognition by inflammasome diversity: Variations on a common themeEuropean Journal of Immunology, 2007
- Variations in serum levels of interleukin (IL)-1β, IL-2, IL-6, and tumor necrosis factor-α during specific immunotherapyAnnals of Allergy, Asthma & Immunology, 2001