Absolute spectrofluorometry

Abstract

The last 10 years has seen the increasing publication of the emission spectra of organic, inorganic and metal-chelate compounds, but there is no agreed method of presentation of such spectra. In the few cases where corrected emission spectra have been published, there is often no mention of the units used for the intensity coordinate or the method used for correcting spectra. A method of reporting absolute fluorescence spectra originally put forward in 1962 will be reexamined and improved. The two best known methods for calibrating spectrometers for absolute spectrofluorometry: (a) standard tungsten lamp, (b) quantum counter method, will be critically examined, and the limitations and possible improvements in accuracy will be proposed. The criteria for an emission standard will be examined and the use of emission standards for calibrating spectrorluorometers discussed. It is suggested that the distribution of emission standards to laboratories measuring corrected fluorescence spectra and the analysis and publication of the results should be done in the near future.