Transcriptional and antagonistic responses of Pseudomonas fluorescens Pf0-1 to phylogenetically different bacterial competitors
- 13 January 2011
- journal article
- Published by Oxford University Press (OUP) in The ISME Journal
- Vol. 5 (6), 973-985
- https://doi.org/10.1038/ismej.2010.196
Abstract
The ability of soil bacteria to successfully compete with a range of other microbial species is crucial for their growth and survival in the nutrient-limited soil environment. In the present work, we studied the behavior and transcriptional responses of soil-inhabiting Pseudomonas fluorescens strain Pf0-1 on nutrient-poor agar to confrontation with strains of three phylogenetically different bacterial genera, that is, Bacillus, Brevundimonas and Pedobacter. Competition for nutrients was apparent as all three bacterial genera had a negative effect on the density of P. fluorescens Pf0-1; this effect was most strong during the interaction with Bacillus. Microarray-based analyses indicated strong differences in the transcriptional responses of Pf0-1 to the different competitors. There was higher similarity in the gene expression response of P. fluorescens Pf0-1 to the Gram-negative bacteria as compared with the Gram-positive strain. The Gram-negative strains did also trigger the production of an unknown broad-spectrum antibiotic in Pf0-1. More detailed analysis indicated that expression of specific Pf0-1 genes involved in signal transduction and secondary metabolite production was strongly affected by the competitors’ identity, suggesting that Pf0-1 can distinguish among different competitors and fine-tune its competitive strategies. The results presented here demonstrate that P. fluorescens Pf0-1 shows a species-specific transcriptional and metabolic response to bacterial competitors and provide new leads in the identification of specific cues in bacteria–bacteria interactions and of novel competitive strategies, antimicrobial traits and genes.Keywords
This publication has 43 references indexed in Scilit:
- GacA-Controlled Activation of Promoters for Small RNA Genes in Pseudomonas fluorescensApplied and Environmental Microbiology, 2010
- The role of natural environments in the evolution of resistance traits in pathogenic bacteriaProceedings Of The Royal Society B-Biological Sciences, 2009
- New developments in microbial interspecies signalingCurrent Opinion in Microbiology, 2009
- Antibiotics and Antibiotic Resistance Genes in Natural EnvironmentsScience, 2008
- Dialogues of root-colonizing biocontrol pseudomonadsEuropean Journal of Plant Pathology, 2007
- Bacterial response regulators: versatile regulatory strategies from common domainsTrends in Biochemical Sciences, 2007
- Cross-Species GacA-Controlled Induction of Antibiosis in PseudomonadsApplied and Environmental Microbiology, 2007
- The Phosphate Starvation Stimulon ofCorynebacterium glutamicumDetermined by DNA Microarray AnalysesJournal of Bacteriology, 2003
- Regulatory Roles of the GacS/GacA Two-Component System in Plant-Associated and Other Gram-Negative BacteriaMolecular Plant-Microbe Interactions®, 2001
- Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionGene, 1989