Cytotoxicity of immune guinea-pig cells. II. The mechanism of macrophage cytotoxicity.

  • 1 April 1973
    • journal article
    • Vol. 24 (4), 655-69
Abstract
Macrophages from immune guinea-pigs, as well as macrophages from non-immune animals in the presence of specific antibody, were cytotoxic to chicken erythrocytes. Elution from macrophages produced a γ2-globulin which enabled non-immune macrophages to form rosettes with chicken erythrocytes, to phagocytose, and to show cytotoxicity towards such cells. This antibody was target-cell specific; it also rendered spleen cells cytotoxic. Isoelectric focusing of antiserum gave a peak of cytophilic antibody at pH 6.8–7.6 which proved to be in the γ2 region.