A Resinous Mounting Medium Compatible with Azo-Dye Staining for Alkaline Phosphatase

Abstract

Thick sections, 75-125 μ, are required for adequate demonstration of blood vessels and occasional other structures which show alkaline phosphatase activity. The mounting medium described has been found to be superior for thick sections to others which we have used such as polyvinylpyrrolidone, polyvinylacetate or glycerol-gelatin. The mounting technique consists of dehydration in absolute ethanol, one change of equal parts of ethanol and cumene, clearing in ethyl benzene, and mounting with synthetic resin dissolved in ethyl benzene.