Synthesis and properties of vinyl monomer/enzyme conjugates
- 1 June 1987
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 15 (1), 11-24
- https://doi.org/10.1007/bf02798503
Abstract
Monomer conjugation of an enzyme followed by copolymerization with free monomer is a useful method of enzyme immobilization. L-asparaginase was conjugated with N-succinimidyl acrylate. Analysis of the conjugated enzyme via isoelectric focusing showed that a molar ratio of 9.5 free monomers per enzyme was needed during the conjunction for each vinyl group bound. Only 3% of the enzyme activity was lost per vinyl group added, and conjugation of an average of four monomers per enzyme thermally destabilized the enzyme only at temperatures above 50‡C. Activity of the enzyme at physiological temperatures was relatively unaffected.Keywords
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