Mapping of a gene for a major outer membrane protein ofEscherichia coli K12 with the aid of a newly isolated bacteriophage

Abstract
A method is described for the enrichment of phages which can adsorb to a specific determinant of bacterial cell surfaces. A phage was isolated which adsorbs toE. coli cells containing the “major outer membrane” proteinc but not to strains that are lacking this protein. With the aid of this phage a gene,meoA which is responsible for the lack of proteinc was mapped at 48 min on the linkage map ofE. coli K12.