Formation of D‐Phe‐Pro‐Val‐cyclo‐Orn by gramicidin S synthetase in the absence of L‐leucine
- 3 March 1987
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 163 (2), 297-302
- https://doi.org/10.1111/j.1432-1033.1987.tb10800.x
Abstract
The preparation of both enzymes of gramicidin S synthetase was efficiently improved by introduction of the fast protein liquid chromatography technique. High-resolution anion-exchange chromatography on Pharmacia Mono Q HR 5/5 was used as the final purification step. D-Phe-Pro-Val-cyclo-Orn was obtained as a product of the multienzyme by omission of L-leucine from the complete bioassay mixture. This tetrapeptide was formed by cyclization of the C-terminal ornithine to 3-amino-2-piperidone. It was identified and characterized by chromatographic and spectroscopic procedures using chemically synthesized reference compounds.This publication has 22 references indexed in Scilit:
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