areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a ς 54 -Dependent Regulator
Open Access
- 1 January 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (1), 6045
- https://doi.org/10.1128/jb.183.1.405-409.2001
Abstract
The areCBA genes in Acinetobacter sp. strain ADP1, determining growth on benzyl alkanoates, are shown to be transcribed as a single operon and regulated by areR , which encodes a regulatory protein of the NtrC/XylR family. Assays of the Are enzymes and of two insertions of lacZ as a reporter gene have shown that the operon is induced by benzyl acetate, benzyl alcohol, and benzaldehyde, as well as 2- and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. Two adjacent sites of transcriptional initiation were 97 and 96 bp upstream of the start codon for areC , near a ς 54 -dependent −12, −24 promoter. Inactivation of areR and rpoN (for RNA polymerase ς 54 ) drastically reduced growth rates on the Are substrates and induction of the operon.Keywords
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