Dependence of in vitro Demineralization of Apatite and Remineralization of Dental Enamel on Fluoride Concentration

Abstract

The anti-caries activity of fluoride is contributed to in several ways. Two major aspects of fluoride action are (i) the inhibition of demineralization at the crystal surfaces within the tooth, and (ii) the enhancement of subsurface remineralization resulting in arrestment or reversal of caries lesions. Fluoride present in the aqueous phase at the apatite crystal surface may play a determining role in the inhibition of enamel or dentin demineralization. In one part of the present study, the initial dissolution rate of synthetic carbonated-apatite in acetate buffers was measured with fluoride present in the buffer in the 0–2.6 mmol/L (0–50 ppm) range. Inhibition of demineralization was shown to be a logarithmic function of the fluoride concentration in solution. In the second part of the present study, an in vitro pH-cycling model was used for determination of the effect on net de/ remineralization of enamel by treatment solutions containing fluoride in the 0–26 mmol/L (0–500 ppm) range. The net mineral loss was shown to be negatively related to the logarithm of the fluoride concentration.