Flow Injection Based Renewable Electrochemical Sensor System
- 1 November 1996
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 68 (21), 3808-3814
- https://doi.org/10.1021/ac960524a
Abstract
A novel class of electrochemical sensors is proposed utilizing electrically conducting beads to form a disposable electrode as well as nonconducting beads to form renewable layers of immobilized enzymes. The concept, aimed to prevent fouling, is tested on an amperometric sensor coupled to nonconducting beads with different immobilized oxidases: galactose, lactate, alcohol, or glucose oxidase, the latter two being used to determine alcohol and gluocse, respectively, in samples of beer and wine. Glucose oxidase was also immobilized on conducting glassy carbon particles to explore the performance of a biosensor where both enzyme and electrode can be automatically renewed in less than 1 min. The results confirm that the concept of a flow injection renewable electrochemical sensor (FI-RES) is practical. It provides a novel approach to biosensing, to comparing enzyme activity, to studying enzyme immobilization on different supports, and to voltammetry in general.Keywords
This publication has 17 references indexed in Scilit:
- In AC Research: In AC ResearchAnalytical Chemistry, 1996
- Characterisation of inhibitors of acetylcholinesterase by an automated amperometric flow-injection systemAnalytica Chimica Acta, 1995
- Amperometric Detection of Peroxides with Poly(anilinomethylferrocene)-Modified Enzyme ElectrodesAnalytical Chemistry, 1995
- Flow injection renewable fibre optic sensor system. Principle and validation on spectrophotometry of chromium(VI)The Analyst, 1995
- Flow Injection Renewable Surface Immunoassay: A New Approach to Immunoanalysis with Fluorescence DetectionAnalytical Chemistry, 1994
- Jet ring cell: a tool for flow injection spectroscopy and microscopy on a renewable solid supportAnalytical Chemistry, 1993
- Comparison between different inorganic supports for the immobilization of amyloglucosidase and α-amylase to be used in enzyme reactors in flow-injection systems: Part I. Hydrolysis of maltose, maltooligosaccharides and soluble starchAnalytica Chimica Acta, 1993
- Comparison between dispersion and plug‐flow models for fixed‐bed enzyme reactorsAIChE Journal, 1992
- Improvement of the performance of porous electrodes using ionic conducting particles: Application to silver recoveryJournal of Applied Electrochemistry, 1990
- Electroanalytical voltammetry in flowing solutionsAnalytica Chimica Acta, 1986