Ionic channel activity induced by fusion ofRhodospirillum rubrumchromatophores with a planar bilayer lipid membrane
- 3 January 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 337 (1), 77-80
- https://doi.org/10.1016/0014-5793(94)80633-0
Abstract
The present work concerns mechanisms of ionic conductivity of photosynthetic membranes. It is shown that reconstitution of vesicles of photosynthetic membranes (chromatophores) of purple bacteria Rhodospirillum rubrum into a planar bilayer lipid membrane leads to fluctuations of current showing the existence of a channel with a predominant conductance of ~ 230 pS in the presence of 100 mM KCl. Measurements under the conditions of KCl gradient prove that this channel is cation selective (Pk/Pcl = 7.2). Voltage inactivation of the channel is demonstrated which is prevented by treatment with trypsinKeywords
This publication has 21 references indexed in Scilit:
- Mitochondrial benzodiazepine receptor linked to inner membrane ion channels by nanomolar actions of ligands.Proceedings of the National Academy of Sciences, 1993
- Purification and patch clamp analysis of a 40-pS channel from rat liver mitochondriaBiochemistry, 1992
- ATP-sensitive K+ channel in the mitochondrial inner membraneNature, 1991
- The inner mitochondrial membrane contains ion‐conducting channels similar to those found in bacteriaFEBS Letters, 1989
- Direct Measurement of K+ Channels in Thylakoid Membranes by Incorporation of Vesicles into Planar Lipid BilayersPlant Physiology, 1989
- A voltage-dependent chloride channel in the photosynthetic membrane of a higher plantNature, 1988
- Patch-clamping of the inner mitochondrial membrane reveals a voltage-dependent ion channelNature, 1987
- Ion Channel ReconstitutionPublished by Springer Nature ,1986
- Conduction and selectivity in potassium channelsThe Journal of Membrane Biology, 1983
- The Development of Electrochemical Potential Gradients across Photosynthetic MembranesPublished by Elsevier ,1982