Effects of tobacco smoke extracts on collagen biosynthesis by fibroblast cell cultures

Abstract
The effects of tobacco smoke extracts on collagen and total protein synthesis by [human skin] fibroblast cell cultures were studied. Tobacco extract with a nicotine concentration of 35 nmol/ml culture medium increased collagen production but not protein secretion in the culture medium. Not only the rate of collagen synthesis but also the types of collagen produced were affected; there was a decrease of type 3 collagen.