Mikrobestimmungen von Purinsubstanzen in Geweben.

Abstract

Guanine is deaminized by purified guanase (from rabbit liver), while adenine does not yield NH3. Tissue enzymes are killed by freezing with liquid air. After hydrolysis with 2% H2SO4 and alkalinization with 33% NaOH the NH3 formed is removed by vacuum distillation. When the last traces of NH3 have been removed, the enzyme soln. is added and the whole kept at 40[degree] C for 3 hrs. at pH 8.8. NH3 formed is then removed in vacuo. The guanine content of rabbit liver, muscle and kidney varies from 0.023 to 0.114%, pig spleen contains 0.165% and calves[image thymus from 0.446 to 0.893%.