A 1H n.m.r. study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductase
Open Access
- 1 February 1984
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 81 (2), 309-315
- https://doi.org/10.1111/j.1476-5381.1984.tb10080.x
Abstract
1 The binding of a series of amide derivatives of methotrexate to Lactobacillus casei dihydrofolate reductase has been studied by inhibition constant measurements and by 1H n.m.r. spectroscopy. 2 Amide modification of the α-carboxylate of methotrexate was found to prevent interaction of the γ-carboxylate with the imidazole of His 28. 3 Estimates of the contributions to the binding energy from the α-carboxylate-Arg 57 and γ-carboxylate-His 28 interactions have been made from a combination of inhibition and n.m.r. data.This publication has 22 references indexed in Scilit:
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