Abstract
A genetic method was devised to test the hypothesis that in some cysteine or methionine requiring (cym) mutants of Salmonella typhimurium suppression of auxotrophy is due to an insertion at the site of the cym mutation. It was found that suppressed strains have an insertion of about 9 kb in the cysCDHIJ region and that in unstable suppressed strains it is the instability of this insertion which results in the segregation of cym auxotrophs.