Comparison of measured growth rates with those calculated from rates of photosynthesis in Planktothrix spp. isolated from Blelham Tarn, English Lake District

Abstract

• Differences in photosynthetic production and conversion to biomass of the red-coloured cyanobacterium Planktothrix rubescens and the green-coloured Planktothrix agardhii , were investigated in relation to their growth in Blelham Tarn, UK, using clonal isolates from the lake. • Growth rates (µ) were measured in cultures under 12 h : 12 h light : dark cycles at 15 irradiances ( E ) in temperatures (Θ) of 10-25°C. Photosynthetic rates ( P ) were measured under the same conditions. • For P. rubescens , µ reached a maximum of 0.33 d ^-1 at 25°C in photon irradiances > 40 µmol m ^-2 s ^-1 and exceeded µ for P. agardhii over the range of temperatures in Blelham Tarn (< 21°C), although not at temperatures > 25°C. In P. rubescens , the dif ference (Δµ) between the growth rate of cell carbon (µ _C ), calculated from P , and µ was only 3% at 10°C but increased with temperature to 30% at 25°C; in P. agardhii , Δµ values were higher at low temperatures and lower at the higher temperatures. • Using algorithms describing the irradiance- and temperature-dependent growth rates and measured values of E and Θ at different depths in Blelham Tarn, it was demonstrated that P. rubescens would outgrow P. agardhii , though the latter might grow better in warmer and shallower lakes. We discuss the problems of modelling phytoplankton growth from measurements of in situ photosynthesis.