The identification of the β2‐microglobulin binding antigen encoded by the human CDID gene
- 1 January 1991
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 21 (1), 71-78
- https://doi.org/10.1002/eji.1830210112
Abstract
Human cluster of differentiation (CD1) is a family of cell surface glycoproteins composed of a 43–49-kDa heavy chain non-covalently associated with β2-microglobulin. Five human CD1 genes have been detected and cloned. Three genes (CD1A, -B and -C) encode the serologically defined CD1a, -b and -c antigens. Thus two genes remain, CD1D and CD1E, whose protein products have not been characterized so far. This report describes how a β-galactosidase-CD1D fusion protein was used to raise specific antisera and a monoclonal antibody against the CD1D gene product. The monoclonal antibody defines a cell surface molecule expressed on a cortical thymocyte cell line and is composed of a 49-kDa heavy chain associated with β2-microglobulin, which is serologically distinct from CD1a.Keywords
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