Synthesis of 2′-Iodo- and 2′-Bromo-ATP and GTP Analogues as Potential Phasing Tools for X-ray Crystallography
- 1 January 1999
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 18 (1), 137-151
- https://doi.org/10.1080/07328319908045600
Abstract
Ara-adenosine (adenine 9-β-D-arabinofuranoside) and ara-guanosine (guanine 9-β-D-arabinofuranoside) are converted into 2′ halogenated ATP and GTP analogues by triflation and subsequent inversion of configuration at C-2′. For the commercially unavailable ara-guanosine a short synthesis starting from guanosine is presented. The nucleotide analogues could serve for the preparation of heavy atom derivatives of ATP- and GTP-binding proteins useful for protein crystal structure determination by MIR/MAD phasing.Keywords
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