Production of antibody against aflatoxin B1
- 1 May 1977
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 33 (5), 1125-1128
- https://doi.org/10.1128/aem.33.5.1125-1128.1977
Abstract
Antibody against aflatoxin B1 was obtained after one multiple-site injection of bovine serum albumin-aflatoxin B1 conjugate into rabbits. The antibody has greatest binding efficiency for aflatoxin B1, less efficiency for B2, G1, and Q1, and least for aflatoxicol, G2, and M1. Sterigmatocystin, coumarin, and 4-hydroxycoumarin did not give a cross-reaction with the antibody. The sensitivity of the binding assay for detection of aflatoxin B1 is in the range of 0.2 to 2.0 ng per 0.5-ml sample. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody titer determination are described.This publication has 8 references indexed in Scilit:
- Production of antibody against ochratoxin AApplied and Environmental Microbiology, 1976
- Aflatoxin B 1 : Specific Antibodies and Their Use in Radioimmunoassay 2 3JNCI Journal of the National Cancer Institute, 1976
- MycotoxinsAnnual Review of Pharmacology, 1975
- Preparation of oestriol-6-(O-carboxymethyl) oxime and oestrone-6-(carboxymethyl) oxime-bovine serum albumin conjugates.1972
- Analytical Methods for MycotoxinsClinical Toxicology, 1972
- Chromatography of Crude Aflatoxins on Adsorbosil-5Journal of AOAC INTERNATIONAL, 1971
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951