Prostaglandin receptors and hormonal actions on water fluxes in cultured canine renal cells (MDCK line).

Abstract

An established cellular line (MDCK) derived from canine kidney showed specific binding for prostaglandin E2, both when the determination of specific binding was carried out in cells attached to a semipermeable support and when this binding was determined in suspended cells in a Ca-free medium. Specific binding for 3H-prostaglandin F2.alpha. was also observed, and it was inhibited by increasing concentrations of non-radioactive PGF2.alpha. in a dose-related pattern. Cross-reactivity between PGE2 binding and PGF2.alpha. was present. Arginine-vasopressin induced an increase in the apical-to-basal water flux when applied to the basolateral surface. No change was observed when the hormone was in contact with the apical surface of the monolayer. Prostaglandin E2 also induced an increase in water flux, with a slower time course than that of arginine-vasopressin. The presence of PGE2 inhibited the increase in water flux induced by antidiuretic hormone [ADH]. MDCK monolayers depict a physiological antagonism between ADH and prostaglandins which is similar to that observed in natural epithelia.