Differences in Fe(II)-N epsilon(His-F8) stretching frequencies between deoxyhemoglobins in the two alternative quaternary structures.
- 1 April 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (4), 2033-2037
- https://doi.org/10.1073/pnas.77.4.2033
Abstract
Resonance Raman spectra have been obtained of the alpha deoxy and beta deoxy subunits within valency hybrid hemoglobins both in the high-affinity (R) and low-affinity (T) structures. Upon conversion from the R to the T structure, the vibrational frequency of the Fe(II)-N epsilon(His-F8) bond changes from 223 to 207 or 203 cm-1 in the alpha deoxy subunit and from 224 to 220 or 217 cm-1 in the beta deoxy subunit. We estimate that the Fe(II)-N epsilon(His-F8) bond is stretched by the R leads to T transition 3 times more in the alpha subunit (0.024 A) than in the beta subunit (0.0085 A) and, accordingly, the strain energy developed in that bond is 8 times larger in the alpha than in the beta subunit. Hence, the oxygen affinity of the alpha and beta subunits may be regulated by different mechanisms.This publication has 40 references indexed in Scilit:
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